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METHOD TO CLASSIFY ENVIRONMENTAL SAMPLES BASED ON MOLD ANALYSES BY QPCR
Citation:
Haugland, R A., T Meklin, M Varma, L J. Wymer, D. G. Dearborn, I. Yike, AND S J. Vesper. METHOD TO CLASSIFY ENVIRONMENTAL SAMPLES BASED ON MOLD ANALYSES BY QPCR. Presented at Bioaerosols Meeting, Saratoga, NY, September 10-12, 2003.
Impact/Purpose:
To understand children's risks from exposure to molds in their environment and to explore risk management options for mitigating those risks.
Description:
A total of 82 quantitative PCR (QPCR) assays were used to identify and quantify different indoor molds in dust samples from the homes of six infants suffering from pulmonary hemorrhage and 26 reference homes in Cleveland, Ohio. No significant difference was seen in the total cell quantities (presumed to be primarily spores and conidia) of these molds in the reference home (RH) and pulmonary hemorrhage home (PHH) samples. The assay groups were categorized based on whether the ratio of measured organisms in the PHH and RH samples were <2 or > 2. Twenty-two groups were identified in the >2 category. The combined cell quantities of these groups were significantly higher in the PHH samples than in the RH samples (p=0.001) and comprised 4% of all detected organisms in the PHH samples.