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MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN A JP-4 FUEL CONTAMINATED SOIL
Citation:
White, D., J. R. Stephen, Y. J. Chang, Y. J. Gan, A. Peacock, S. J. MacNaughton, S. M. Pfiffner, AND M. J. Barcelona. MOLECULAR CHARACTERIZATION OF MICROBIAL COMMUNITIES IN A JP-4 FUEL CONTAMINATED SOIL. The Fifth International In Situ & On-Site Bioremediation Symposium, San Diego, CA, 4/19-22/1999.
Description:
In this study, lipid biomarker characterization of the bacterial and eukaryotic communities was combined with PCR-DGGE analysis of the eubacterial community to evaluate correlation between JP-4 fuel concentration and community structure shifts. Vadose, capillary fringe and saturated soils were taken from cores within, up- and down-gradient of the contaminant plume. Significant differences in biomass and proportion of Gram negative bacteria were found inside and outside the plume. Sequence analysis of DGGE bands from within the spill site suggested dominance by a limited number of phylogenetically diverse bacteria. Together with pollutant quantification, these molecular techniques should facilitate significant improvements over current assessment procedures for determination of remediation end points.