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POLYCLONAL ANTISERA AGAINST ESTUARINE CRUSTACEAN VITELLINS: A MOLECULAR APPROACH TO REPRODUCTIVE ENDOCRINOLOGY AND TOXICOLOGY.
Citation:
Tuberty, S. R., S. F. Nates, AND C L. McKenney Jr. POLYCLONAL ANTISERA AGAINST ESTUARINE CRUSTACEAN VITELLINS: A MOLECULAR APPROACH TO REPRODUCTIVE ENDOCRINOLOGY AND TOXICOLOGY. Crustacean Society 2000 Summer Meeting, Puerto Vallarta, MEXICO, June 26 - 30, 2000.Kluwer Academic/Plenum Publishers, New York, NY, 29-37, (2002).
Impact/Purpose:
In this study, we developed a two-step chromatographic method to purify Vtfrom six importantcrustaceanspecies.
Description:
To fully elucidate the action of crustacean hormones, or their agonists, on vitellogenesis and reproduction, it has become increasingly important to develop sensitive assays that indicate a stimulatory or inhibitory effect on easily measured endpoints. Because of the relative abundance of vitellin in crustacean yolk and the ease with which it can be isolated, this protein makes an excellent model for studying the mechanisms that control and regulate reproduction. Vitellin was purified from eggs of adult female Lepidophthalmus louisianensis, Palaemonetes pugio, Rhithropanopeus harrisii, Americamysis (Mysidopsis) bahia, and Uca panacea which were collected from estuarine localities in Santa Rosa Sound, Gulf Breeze, Florida during late spring and summer of 1999. The purified proteins were used to immunize rabbits for polyclonal antibody production. Specificity of antisera was tested against respective vitellins by Western blotting. These antisera will be used to develop assays useful to the study of reproductive endocrinology, vitellogenesis, and toxicology.