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ENVIRONMENTAL TECHNOLOGY VERIFICATION REPORT, PCB DETECTION TECHNOLOGY, HYBRIZYME DELFIA TM ASSAY
Citation:
Dindal, A. B., C. K. Bayne, R. A. Jenkins, AND E N. Koglin. ENVIRONMENTAL TECHNOLOGY VERIFICATION REPORT, PCB DETECTION TECHNOLOGY, HYBRIZYME DELFIA TM ASSAY. U.S. Environmental Protection Agency, Washington, DC, EPA/600/R-01/052 (NTIS PB2002-100442), 2001.
Impact/Purpose:
The objective of this task is to increase the acceptance and use of site characterization and monitoring technologies by providing verified performance data and reports on the performance of environmental monitoring and site characterization technologies.
Description:
The DELFIA PCB Assay is a solid-phase time-resolved fluoroimmunoassay based on the sequential addition of sample extract and europium-labeled PCB tracer to a monoclonal antibody reagent specific for PCBs. In this assay, the antibody reagent and sample extract are added to a strip of microtiter plate wells and allowed to react. The strips have been specially treated to trap the antibody reagent or antibody-PCB complexes that may have formed. A wash step removes sample matrix from the captured antibody. This step significantly reduces any potential matrix interferences before the addition of the PCB tracer, resulting in an unusually robust assay system. The PCB tracer is then added and allowed to bind to the antibodies that are not complexed with sample PCBs. A wash step is used to separate antibody-bound tracer from the tracer free in solution. The addition of an enhancement solution forms highly fluorescent chelates with the bound europium ions. The amount of fluorescence measured is inversely proportional to the concentration of PCBs in the sample. The lowest reporting level is typically 0.5 ppm.