You are here:
COMPARATIVE ANALYSIS OF REACTIVE OXYGEN SPECIES IN HUMAN PLASMA AND BLOOD
Citation:
Sams, R. L., C Carty, M Schmitt, J Inmon, S Rhoney, E E. Hudgens, R L. Calderon, AND J Gallagher. COMPARATIVE ANALYSIS OF REACTIVE OXYGEN SPECIES IN HUMAN PLASMA AND BLOOD. Presented at Society of Toxicology 42nd Annual Meeting, Salt Lake City, Utah, March 9-13, 2003.
Description:
Reactive oxygen species (ROS) are commonly associated with diseased states (including asthma, cardiovascular disease, cancer) infections, and exposure to various toxicants in humans. It is of interest in epidemiology studies to characterize the association of oxidative stress in relation to health/exposure status to ascertain which biological media, plasma or whole blood, is a more sensitive measure of ROS. In our study we evaluated differences between plasma and whole blood in the levels of ROS-induced chemiluminescence (using lucigenin as a chemiluminescence agent) measured simultaneously from 194 human subjects over a 3-week study. Mean ROS-induced chemiluminescence measured in plasma (590.8 ? 193.6; mean ? SD; Relative Luminescent Units (RLU)) was approximately 2-fold greater (significant at p = 0.001) in comparison to whole blood (280.7 ? 204.6; RLU). Although whole blood ROS levels were less than plasma ROS levels, we did observe that the inter-individual variation was greater for whole blood compared to plasma. These data suggest that human plasma is a more sensitive biological matrix to measure the levels of ROS-induced chemiluminescence, but due to the greater variability in ROS levels measured in whole blood, we have an opportunity to assess associations between ROS levels and modulators of ROS including diet, age, gender, and smoking status. This abstract does not necessarily represent USEPA policy.