You are here:
RECOVERY OF MUTAGENICITY FROM DISINFECTED WATER SAMPLES BY XAD RESIN ADSORPTION COMPARED TO REVERSE OSMOSIS
Citation:
Schenck*, K M., T F. Speth*, R J. Miltner*, M Sivaganesan*, AND J E. Simmons. RECOVERY OF MUTAGENICITY FROM DISINFECTED WATER SAMPLES BY XAD RESIN ADSORPTION COMPARED TO REVERSE OSMOSIS. Presented at Environmental Mutagen Society Annual Meeting, Anchorage, AK, 4/27-5/2/2002.
Description:
the presence of a complex misture of disinfection byproducts in drinking waters continues to cause concern over the potential long-term health effects of exposure to these compounds. Most of these compounds are present at ug/L levels or less. Consequently, some method of concentration is usually necessary prior to toxicological testing. XAD resin adsorption has been widely used to concentrate the organic compounds present in drinking waters prior to mutagenicity testing. However, this method is not compatible with many other assays, due in part to the fact that the compounds are dissolved in an organic solvent instead of a water matrix. A study was conducted to compare reverse osmosis (RO) filtraion to XAd adsorption for concentrating drinking water samples At a pilot-scale plant, a clarified lake water was disinfected with either ozone followed by chlorine or chlorine alone. Concentrates were prepared by XAD adsorption/ethyl acetate elution or RO filtration. The concentrates were tested in the Ames assay using TA100 and TA98, +/- S9. Both the XAD and RO concentrates showed higher levels of activity in TA100 and the addition of S9 resulted in decreased levels of mutagenicity. The water sample disinfected with chlorine alone had a higher level of activity than the sample treated with ozone followed by chlorine regardless of the concentration method used. These observations are consistent with previous results for XAD concentrates of water samples. However, the levels of mutagenicity observed in the RO samples were significantly less than those in the XAD samples (P<0.05). The data suggest that the mutagenic charcteristics of the compounds recovered by the two methods are similar, however, the recovery of mutagenic compounds appears to be greater by XAD adsorption compared to RO filtration.