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ENHANCED RETENTION AND SENSITIVITY IN THE ANALYSIS OF CYANURIC ACID IN WATER USING POROUS GRAPHITIC CARBON AND UV DETECTION IN HIGH PRESSURE LIQUID CHROMATOGRAPHY
Citation:
Cantu, R, O M. Evans, F K. Kawahara, AND A P. Dufour. ENHANCED RETENTION AND SENSITIVITY IN THE ANALYSIS OF CYANURIC ACID IN WATER USING POROUS GRAPHITIC CARBON AND UV DETECTION IN HIGH PRESSURE LIQUID CHROMATOGRAPHY. Presented at American Chemical Society 220th National Meeting, Washington, DC, August 20-24, 2000.
Impact/Purpose:
Develop a method to determine the volume of recreational water ingested by relating the quantity imbibed to the level of a chemical biomarker measured in urine.
Description:
Cyanuric acid (CA) has found application as a chlorine stabilizer in pool waters. The National Swimming Pool Foundation recommends CA levels between 30-50 ppm and a chlorine residual of 1.0-3.0 ppm. These chlorine levels are needed to destroy harmful pathogenic organisms. Developing a rugged method to monitor CA in water is crucial in maintaining adequate chlorine levels that do not pose undue hazards to human health. Existing methodology employing HPLC has proven ineffective because of lack of CA retention imposed by use of silica based columns. A rugged method has been developed using porous graphitic carbon (PGC) to analyze real world water samples. The analysis employed 955 phosphate:5% methanol at pH 7.4 with UV detection at 213 nm. The CA retention factor (K') using PGC was 8 while for Cl8, C8, C6H5, NH2, and CN silica columns it was unsuitable (<0.1). Raising the pH to 9.2 resulted in practical retention (k'=4) and gave 20% more sensaitivity due to optimum UV detection.