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EFFECTS OF TEMPERATURE AND SOLVENT COMPOSITION ON THE CHIRALCEL OJ SEPARATION OF CHIRAL ORGANOPHOSPHORUS PESTICIDES
Citation:
Ellington, J J. AND J J. Evans. EFFECTS OF TEMPERATURE AND SOLVENT COMPOSITION ON THE CHIRALCEL OJ SEPARATION OF CHIRAL ORGANOPHOSPHORUS PESTICIDES. Presented at 22nd Annual Society of Environmental Toxicology and Chemistry Meeting, Baltimore, MD, November 11-15, 2001.
Impact/Purpose:
Elucidate and model the underlying processes (physical, chemical, enzymatic, biological, and geochemical) that describe the species-specific transformation and transport of organic contaminants and nutrients in environmental and biological systems. Develop and integrate chemical behavior parameterization models (e.g., SPARC), chemical-process models, and ecosystem-characterization models into reactive-transport models.
Description:
The separation of the enantiomers of twelve organophosphorus pesticides (OPs) was investigated on the CHIRALCEL?OJ column to determine whether the mobile phase composition, flow rate and column temperature could be optimized to yield at least partial separation of the enantiomers of the 12 OPs. Chromatographic conditions were determined that gave either baseline or near baseline separations of the enantiomers of the twelve OPs on the CHIRALCEL?OJ column. The separation of the enantiomers of eight OPs improved when the mobile phase content of the polar modifer was decreased to 4% or less and for one OP (trichloronate) to 0.0%. Column efficiency (resolution) increased for the enantiomers of two OPs at 40 0C and the peak 2/peak 1 area ratios decreased from1.2 to nearly unity. The retention times were decreased by 50% for one pair of enantiomers when the column temperature was increased from 20 0C to 40 0C.