You are here:
IN VIVO MEASUREMENT OF PHENYLGLUCUCURONIDE IN RAINBOW TROUT BY ON-LINE INJECTION MICRODIALYSIS
Citation:
Hoffman, A D., C. T. Jenson, G J. Lien, J. W. Nichols, AND J M. McKim. IN VIVO MEASUREMENT OF PHENYLGLUCUCURONIDE IN RAINBOW TROUT BY ON-LINE INJECTION MICRODIALYSIS. Presented at Society of Toxicology National Meeting, San Francisco, CA, March 25-29, 2001.
Description:
Phenylglucuronide (PG) was measured in vivo in arterial blood of rainbow trout using on-line injection microdialysis. A microdialysis probe was surgically implanted in the dorsal aorta of spinally-transected trout. The trout were dosed continuously with PG for 24 h using a ventral aortic cannula. Fish were then allowed to depurate PG for an additional 48 h. The concentration of PG in arterial blood was determined by injecting probe dialysate directly onto a Beckman HPLC equipped with a diode array detector. Instrument conditions gave a sample run tie of 16 min and a lower detection limit of 5 uM. The minimum sampling interval was 30 min, including the time to flush the on-line injector. Para-nitrophenylsulfate was used as an internal standard to provide in vivo sample-to-sample probe recovery values. In vivo recoveries were always lower than in vitro (stirred) recoveries and tended to decline with time, possibly due to clot formation around the probe. The techniques developed in this study offer significant advantages over periodic blood sampling for kinetic studies with fish including automated sample collection, near real-time analysis of protein-free samples, potential application to volatile or easily degraded compounds, minimal blood loss, and reduced sampling stress.