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SIMPLE SAMPLE CLEAN UP PROCEDURE AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF CYANURIC ACID IN HUMAN URINE
Citation:
Cantu, R, O M. Evans, T D. Behymer, J A. Shoemaker, A P. Dufour, AND F K. Kawahara. SIMPLE SAMPLE CLEAN UP PROCEDURE AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF CYANURIC ACID IN HUMAN URINE. Presented at ACS Meeting, San Diego, CA, April 1-5, 2001.
Impact/Purpose:
Develop a method to determine the volume of recreational water ingested by relating the quantity imbibed to the level of a chemical biomarker measured in urine.
Description:
Cyanuric acide (CA) is widely used as a chlorine stabilizer in outdoor pools. No simple method exists for CA measurement in the urine of exposed swimmers. The high hydrophilicity of CA makes usage of solid phase sorbents to extract it from urine nearly impossible because of sample breakthrough. However, octadecyl silica cartridges retain most urinary interferences while allowing CA to pass through unretained. Using this simple approach, CA recoveries of 89-112% were obtained using isocratic high performance liquid chromatographic conditions (graphitic carbon column, 95% phosphate buffer: 5% methanol eluent, and UV detection at 213 nm). The resulting method offers effective CA separation from urinary interferences including creatinine (identified by electrospray ionization mass spectrometry), and detectability of 0.02 mg CA per liter of urine. The acidic nature of CA permits its preservation in urine for 3 weeks through the addition of 1% perchloric acid and 0.1% metaphosphoric acid.