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ENZYME DEGRADATION OF CHIRAL ORGANIC PHOSPHORUS INSECTICIDES
Citation:
Ellington, J J., J J. Evans, AND S C. Cook. ENZYME DEGRADATION OF CHIRAL ORGANIC PHOSPHORUS INSECTICIDES. Presented at 20th Annual Society of Environmental Toxicology and Chemistry Meeting, Philadelphia, PA, November 14-18, 1999.
Description:
Chiral organic phosphorus pesticides (OPs) are expected to be biologically degraded enantioselectively by endogenous enzymes. Various chiral Ops were treated with the enzyme phosphotriesterase (PTE) obtained from partially purified extracts of Escherichia coli strain DH-5- carrying a cloned opd gene from Flavohacterium. The enzymatic degradation of the OP enantiomers was followed by HPLC using a Chiraicel OJ chiral column. Initial results, using the OP Crotoxyphos, indicated that each enantiomer of Crotoxyphos degraded at a different rate. UV-Vis spectrometry was used to determine the overall rate constant for the racemic mixtures.