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DETECTION OF DNA DAMAGE USING A FIBEROPTIC BIOSENSOR
Citation:
Rogers, K R., A. B. Apostol, S. J. Madsen, AND C. W. Spencer. DETECTION OF DNA DAMAGE USING A FIBEROPTIC BIOSENSOR. Presented at ACS National Meeting, San Francisco, CA, March 26-30, 2000.
Impact/Purpose:
The overall objective of this task is to develop rapid, cost-effective and scientifically sound techniques for measuring chemically induced DNA damage. This method is expected to provide the Agency with rapid, sensitive, and simple techniques that can be used among a panel of methods to determine the genotoxic potential of polluted samples.
Description:
A rapid and sensitive fiber optic biosensor assay for radiation-induced DNA damage is reported. For this assay, a biotin-labeled capture oligonucleotide (38 mer) was immobilized to an avidin-coated quartz fiber. Hybridization of a dye-labeled complementary sequence was observed using the evanescent wave and reported at the proximal end of the optical fiber. Changes ire temperature-induced strand separation were then used as a sensitive indicator of damage resulting from exposure of the dye-labeled strand to low doses of ionizing radiation. Ten assays (each requiring about 15 main) could be run. on the same fiber. Radiation exposures of between 0.2 and 10 Gray (Gy) were measured.
The U.S. Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), funded this research and approved this abstract as a basis for an oral presentation. The actual presentation has not been peer reviewed by EPA.