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CHARACTERIZATION OF A MOUSE MODEL OF IIMMUNOSUPPRESSION USING A SINGLE DOES OF METHYLPREDNISOLONE ACETATE
Citation:
Miller, T A. AND F W. Schaefer. CHARACTERIZATION OF A MOUSE MODEL OF IIMMUNOSUPPRESSION USING A SINGLE DOES OF METHYLPREDNISOLONE ACETATE. Presented at Society of Comparative Endocrinology, Asheville, NC, June 1-4, 2003.
Impact/Purpose:
1) Refine new, practical methods for the detection of CCL-related and emerging waterborne human protozoa.
2) Perform field tests of devices or methods that have been developed under this task.
3) Evaluate these methods or devices in a variety of water matrices and parasite concentrations.
This work in this task supports CCL2 and 3 and is expected to be completed by 9/07.
Description:
Immunosuppression is a widely used but rarely defined term. To the transplant specialist it is the state at which the transplanted organ is not rejected. For the HIV patient it is the point when AIDS begins. In research, immunosuppression is achieved through the use of glucocorticoids, usualy for prolong period of time. In this model a single dose of methylprednisolone acetate (Depomedrol) was used to produce immunosuppression. The circulating CD3, CD4 and CD8 lymphocyte levels were analyzed to characterize the immunologic changes induced. A drop of greater than 80% was needed to re-establish a Cryptosporidium muris infection in mice who had fully recovered from an initial infection. When compared, the ID50 for mice who were immunosuppressed was three times higher than the ID50 for normal mice, showing immunosuppressed mice were less susceptible to C. muris invasion.