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ENHANCED RETENTION AND SENSITIVITY IN THE ANALYSIS OF CYANURIC ACID IN WATER USING POROUS GRAPHITIC CARBON AND UV DETECTION IN HPLC
Citation:
Cantu, R, O M. Evans, F K. Kawahara, AND A P. Dufour. ENHANCED RETENTION AND SENSITIVITY IN THE ANALYSIS OF CYANURIC ACID IN WATER USING POROUS GRAPHITIC CARBON AND UV DETECTION IN HPLC. Presented at American Chemical Society, Covington, KY, 05/16-19/2000.
Description:
Cyanuric acid (CA) has found application as a chlorine stabilizer in pool waters. The National Swimming Pool Foundation recommends CA levels between 30-50 ppm and a chlorine residual of 1.0-3.0 ppm. These chlorine levels are needed to destroy harmful pathogenic organisms. Developing a rugged method to monitor CA in water is crucial in maintaining adequate chlorine that do not pose undue hazards to human health. Existing methodology employing HPLC has proved ineffective because the lack of CA retention imposed by the use of silica based columns. A rugged method has been developed using CA retention imposed by the use of silica based columns. A rugged method has been developed using porous graphitic carbon (PGC) tp analyze real world water samples. The analysis employed 95% phosphate, 5% methanol at pH 7.4 with UV detection at 213nm. The CA retention factor (k') using PGC was 8 while for C18, C8, C6H5, NH2, and CN silica columns it was unsuitable (<0.1), Raising the pH to 9.2 resulted in practical retnetion (K'+4) and gave 20% more sensitivity due to optimum UV detection.