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STUDIES OF NORMAL GENE EXPRESSION IN THE RAT NASAL EPITHELIUM USNG CDNA ARRAY TECHNOLOGY
Citation:
Hester, S D., G. R. Benavides, M. Sartor, L. Yoon, K. T. Morgan, AND D C. Wolf. STUDIES OF NORMAL GENE EXPRESSION IN THE RAT NASAL EPITHELIUM USNG CDNA ARRAY TECHNOLOGY. Presented at AACR Oncogenomics, Tucson, Arizona, January 25-28, 2001.
Description:
Studies of Normal Gene Expression in the Rat Nasal Epithelium Using cDNA Array
The nasal epithelium is an important target site for chemically-induced toxicity and carcinogenicity .Gene expression data are being used increasingly for studies of such conditions. In order to provide normal baseline data for this target organ, we investigated gene expression profiles in nasal transitional/respiratory epithelium of healthy rats. Cells lining the anterior nasal passages, as confirmed by light microscopy, were collected into 200 ul of TrizolTM reagent by controlled intranasal instillation. Gene expression analysis was performed using Clontech TM cDNA Rat Atlas 1.2 arrays (~1200genes) and selected transcripts verified using RT PCR (TaqMan TM). The percentages of genes within specific average expression ranges, based on phosphorimager signal intensities, were 1% at 3000-45,000; 3% at 1000-3000; 30% at 100-1000; 65% below 100. The most highly expressed genes included those involved in phase I (e.g. CYPs) and phase II (e.g. GSTs) xenobiotic metabolism, bioenergetics (e.g. cytochrome oxidase), osmotic balance (e.g. Na+/K+ ATPase) and epithelial ionic homeostasis (e.g. ion channels). These findings are consistent with normal nasal epithelial functions. Such baseline data will contribute to further understanding of the functions of this region of the respiratory tract and facilitate the interpretation of treatment or disease-related responses by the nasal epithelial transcriptome.
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