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CURRENT METHODS FOR DETECTION OF CRYPTOSPORIDIUM SPECIES
Citation:
Lindquist, H.D A. CURRENT METHODS FOR DETECTION OF CRYPTOSPORIDIUM SPECIES. Presented at Science and Mission Club, Cincinnati, OH, November 8, 2000.
Impact/Purpose:
1) Refine new, practical methods for the detection of CCL-related and emerging waterborne human protozoa.
2) Perform field tests of devices or methods that have been developed under this task.
3) Evaluate these methods or devices in a variety of water matrices and parasite concentrations.
This work in this task supports CCL2 and 3 and is expected to be completed by 9/07.
Description:
Current methods for detecting protozoa in water produce results that are highly variable. It is difficult to determine if the methods themselves, or the procedures for testing these methods, are the source of the variability. If testing procedures are responsible for high variability, then the way in which methods are tested may not provide a fair comparison. To overcome this problem, a set of criteria has been developd to aid in the evaluation of detection methods for parasites in water. Application of these criteria has elucidated some of the most important causes of testing variability. Approaches have been developed to overcome these pitfalls. Comparisons of several methods for detecting Cryptosporidium spp. in the environment, using more precise techniques, indicate differences in the efficiency of these methods.