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MULTIPLE EFFECTS OF ETHANE DIMENTHANESULFONATE ON THE EPIDIDYMIS OF ADULT RATS
Citation:
Klinefelter, G., J. Laskey, N. Roberts, V. Slott, AND J. Suarez. MULTIPLE EFFECTS OF ETHANE DIMENTHANESULFONATE ON THE EPIDIDYMIS OF ADULT RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/284 (NTIS PB91144691), 1990.
Description:
Ethane dimethanesulphonate (EDS), a compound cytotoxic to Leydig cells which causes transient infertility, was used in a 4 d post-exposure experimental protocol designed to identify any effects this compound might exert on the epididymis. he techniques efferent duct ligation and testosterone(T) implantation were used to negate the role of testicular effects on the epididymal parameters. umerous evaluations were performed including light and electron microscopy, assay of steroid hormones in serum and epididymal tissue, computer assisted sperm motion analyses, and electrophoresis sperm membrane proteins. DS was shown to affect the epididymis in a dose dependent fashion. he action of EDS on the epididymis is in part due to the androgen deprivation caused by the elimination of Leydig cells in the testis since T implantation prevented some of the morphological changes in the epididymis, as well as some of the changes in sperm proteins and motility. owever, neither efferent duct ligation nor T implantation prevented the formation of sperm granulomas in the caput epididymidis, the distinct morphological alterations of the corpus epididymidis, the modification of certain sperm membrane proteins, or the decrease in the progressive motility and velocity of sperm following EDS treatment. lthough we can not prove these effects of EDS are due to a direct action on the epididymis, it is now clear that EDS has distinct action on the epididymis which is unrelated to circulating T or testicular fluid.
