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QUANTITATIVE 15N NMR SPECTROSCOPY
Citation:
Levy, G., T. Pehk, AND P. Srinivasan. QUANTITATIVE 15N NMR SPECTROSCOPY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-80/380 (NTIS PB82155250), 1980.
Description:
Line intensities in 15N NMR spectra are strongly influenced by spin-lattice and spin-spin relaxation times, relaxation mechanisms and experimental conditions. Special care has to be taken in using 15N spectra for quantitative purposes. Quantitative aspects are discussed for the 15N NMR of molecules with different nitrogen functional groups and also mixtures of nitrogen-containing compounds. It is shown that, in general, quantitative data are obtainable from integration of 15N lines in proton decoupled 15N NMR spectra using NOE suppression. Addition of paramagnetic relaxation reagents (PARR) under controlled conditions is frequently needed to accomplish the experiment within reasonable time limits.