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ULTRAVIOLET RADIATION-INDUCED NEOPLASTIC TRANSFORMATION OF NORMAL HUMAN CELLS, IN VITRO
Citation:
Milo, G., S. Weisbrode, R. Zimmerman, AND J. McCloskey. ULTRAVIOLET RADIATION-INDUCED NEOPLASTIC TRANSFORMATION OF NORMAL HUMAN CELLS, IN VITRO. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-81/626.
Description:
Human foreskin cell cultures in schedules DNA synthesis (S phase) of the cell cycle were exposed to UV irradiation at a dose of 10 J.sq. m. in the presence of insulin. These treated cell populations, when selectively passaged in a high amino acid supplemented complete growth medium (CM) after 20 Dulbecco's phosphate buffered saline (pH 6.8) (PDL), were able to be grown in soft agar. These treated cell populations were also grown in 1% serum supplemented growth medium and at 41 C in 10% serum supplemented growth medium.
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ULTRAVIOLET RADIATION-INDUCED NEOPLASTIC TRANSFORMATION OF NORMAL HUMAN CELLS, IN VITRORecord Details:
Record Type:DOCUMENT( REPORT )
Product Published Date:05/24/2002
Record Last Revised:04/16/2004
Record ID:
51435