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SELECTION KINETICS DURING SERIAL CELL CULTURE PASSAGE OF MIXTURES OF WILD TYPE AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS AND ITS RECOMBINANT AC360-B-GAL
Citation:
Huang, Y., K. Bobseine, R. Setzer, AND C. Kawanishi. SELECTION KINETICS DURING SERIAL CELL CULTURE PASSAGE OF MIXTURES OF WILD TYPE AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS AND ITS RECOMBINANT AC360-B-GAL. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/015 (NTIS PB92143874).
Description:
Detailed analysis of the selection process in serial co-infections of cell cultures by wild type Autographa californica nuclear polyhedrosis virus, AcNPV/E2, and Ac36O-B-gal, a "genetically engineered" strain, shows that the unaltered strain was clearly dominant even when it began as the minority component in the inoculum. ethod of calculating a selection coefficient that quantifies the relative advantage of one strain of virus over the other under specific culture conditions is described. alculated selection coefficients were relatively homogeneous and almost exclusively favored the progenitor. election pressure was uninfluenced by the relative proportions of the two strains in the population. election coefficients, as determined in the present study, may be useful for evaluating the effect of a genetic alteration on viral fitness under specified conditions. nexpected high frequencies of mixed phenotype plaques were observed during infectivity titrations of media from early serial passages of coinfected cultures. tatistical evaluation implicates some nonheritable combinational phenomenon virus plated from mixed phenotype plaques show high segregation of phenotypes implying that genetic recombination does not contribute in a major way to the high mixed phenotype frequencies.