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(32)P-POSTLABELING ANALYSIS OF DNA ADDUCTS OF TWO NITRATED POLYCYCLIC AROMATIC HYDROCARBONS IN RABBIT TRACHEAL EPITHELIAL CELLS
Citation:
Gallagher, J., I. Robertson, M. Jackson, A. Dietrich, AND L. Ball. (32)P-POSTLABELING ANALYSIS OF DNA ADDUCTS OF TWO NITRATED POLYCYCLIC AROMATIC HYDROCARBONS IN RABBIT TRACHEAL EPITHELIAL CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-87/240 (NTIS PB88103718).
Description:
The 1-nitropyrene (1-NPP and 3-nitrofluoranthene (3-NF) adducts have been analyzed by (32)P-postlabeling and with 1-NP have been compared to the total number of adducts estimated from (14)C binding in rabbit trachael epithelial (RTE) DNA samples. One adduct spot, by (32)P-postlabeling, was detected in the DNA of RTE cells treated with (14)C-1-NP. The adduct, at approximately 6 adducts 10-to the 9th power nucleotides, represents a minor component of the binding to RTE DNA being lesser than 1% of the total (14)C-radioactivity associated with the extracted DNA. Treatment of RTE cells with unlabeled 3-NF resulted in two adduct spots at approximately 12 adducts/10 to the 9th power. When the P1 nuclease treatment was used as an alternative method both the 3-NF derived adducts previously detected following the butanol extraction were absent.