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INFLUENCE OF CYTOCHROME P-450 MIXED IN FRACTIONS OXIDASE INDUCTION ON THE ACUTE TOXICITY TO RAINBOW TROUT (SALMO GAIRDNERI) OF PRIMARY AROMATIC AMINES
Citation:
Hermens, J., S. Bradbury, AND S. Broderius. INFLUENCE OF CYTOCHROME P-450 MIXED IN FRACTIONS OXIDASE INDUCTION ON THE ACUTE TOXICITY TO RAINBOW TROUT (SALMO GAIRDNERI) OF PRIMARY AROMATIC AMINES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/391 (NTIS PB91171470), 1990.
Description:
The influence of enzyme induction on acute toxicity of aniline and 4-chloroaniline to rainbow trout (Salmo gairdneri) was investigated. or these two xenobiotics, bioactivation reaction are known to occur in mammals. nduction of cytochrome P450 mixed-function oxidase was obtained by intraperitoneal (ip) injection of trout with a mixture of polychlorinated biphenyls (Aroclor 1254). ive days after ip injection with three different does of Aroclor 1254 (50, 100, and 200 mg/kg), benzo[a]pyrene hydroxylase activity in trout liver microsomes increased five- to sixfold. ytochrome P450 concentrations in the microsomes were slightly, but significantly, enhanced in two of the three dose levels. he 96-hr LC50's of aniline and 4-chloroaniline were not affected by pretreatment with Aroclor 1254, suggesting that metabolic activation does not necessarily play a role in the acute toxicity of aromatic amines to fish.
