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CATABOLISM OF AROMATIC BIOGENIC AMINES BY 'PSEUDOMONAS AERUGINOSA' PA01 VIA META CLEAVAGE OF HOMOPROTOCATECHUIC ACID (JOURNAL VERSION)
Citation:
Cuskey, S. AND R. Olsen. CATABOLISM OF AROMATIC BIOGENIC AMINES BY 'PSEUDOMONAS AERUGINOSA' PA01 VIA META CLEAVAGE OF HOMOPROTOCATECHUIC ACID (JOURNAL VERSION). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/109 (NTIS PB89120067).
Description:
Pseudomonas aruginosa PA01 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). Meta ring cleavage was mediated by 3-4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-dependent dehydrogenase caused the disappearance of the yellow MSA product, probably forming 2-hydroxy-5-carboxymethylmuconic acid. Induction studies with extracts from mutant cells indicated that the inducer of HPADO was HPA and/or MSA. Strains PA01.221 (tynCl) and PA01.303 (tynDI) have chromosomal mutations causing a deficiency in the activity necessary for conversion of 4-hydroxyphenylacetic acid to HPA. (Copyright (c) 1988 American Society for Microbiology.)