You are here:
EFFECTS OF ETHANE DIMETHANESULFONATE (EDS) ON ADULT AND IMMATURE RABBIT LEYDIG CELLS: COMPARISON WITH EDS-TREATED RAT LEYDIG CELLS
Citation:
Laskey, J., G. Klinefelter, W. Kelce, AND L. Ewing. EFFECTS OF ETHANE DIMETHANESULFONATE (EDS) ON ADULT AND IMMATURE RABBIT LEYDIG CELLS: COMPARISON WITH EDS-TREATED RAT LEYDIG CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/054.
Description:
Ethane-dimethanesulfonate (EDS) has been shown to selectively kill Leydig cells and depress testosterone production in adult rats. ecent study has shown that immature rat leydig cells are less sensitive to EDS exposure. here is evidence that the rabbit metabolizes EDS to methane sulfonic acid more rapidly than the rat. his study examines the effects of EDS on the leydig cells in both adult and immature abbits and compares the effects found with those previously reported in the rat. In vivo EDS exposure demonstrated that Leydig cells from adult rabbits were affected, ith both serum and interstitial testosterone production depressed. DS effects in adult rabbits and rats were compared using EDS exposure to quarter testis and evaluating testosterone production. ith this procedure the rabbit testis was less sensitive to EDS treatment than the rat with EC50's of 2026 uM and 336 uM, respectively. erfusion of adult and immature rabbit testis with 430 uM EDS demonstrated the insensitivity of the immature testis to EDS exposure with adult testosterone production reduced 50% in 3.5 hours, while no demunition was found in the immature rabbit. DS exposure of interstitial cell preparations further demonstrated the insensitivity of the immature rabbit Leydig cells with an EC50 of 4397 uM compared to an EC50 of 1137 uM in adult preparations. hese and previously reported data in the rat show that Leydig cell sensitivity to EDS exposure is ordered; adult rat > adult rabbit > immature rat >> immature rabbit.