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CELL GROWTH IN PLANT CULTURES: AN INTERPRETATION OF THE INFLUENCE OF INITIAL WEIGHT IN CADMIUM AND COPPER TOXICITY TESTS
Citation:
Shirazi, M., M. Robideaux, L. Kapustka, J. Wagner, AND M. Reporter. CELL GROWTH IN PLANT CULTURES: AN INTERPRETATION OF THE INFLUENCE OF INITIAL WEIGHT IN CADMIUM AND COPPER TOXICITY TESTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/468 (NTIS PB95137303), 1994.
Description:
The authors present an approach for conducting and interpreting results of newly established plant cell culture in toxicity studies. xtended culturing produces uniform suspension and facilities sampling. rimary (new) cultures are more representative of all responses of their plant species but produce nonuniform suspension due to clumping and clumping presents unavoidable sampling difficulties. valuations were made of the response of plant cell suspension in primary cultures subjected to etal stress in three different experiments. ampling and data interpretation were examined in detail, using mathematical models. ells were selected from callus cultures of Medicago sativa and green Mandevilla pohliana for experiments using copper and from non-green Mandevilla pohliana for an experiment using cadmium. istributions of as many as 21 elements in soluble and cell-residue compartments were determined. he interaction of treatment with copper and element distribution was emphasized. ulture status was traced microscopically and growth measured as change in cell fresh weight. he initial weight of each culture replicate could not be held constant due to nonuniform suspension. athematical model that described the interactions of the initial weight, exposure time, and metal concentrations was developed to analyze the data. he model revealed stimulation of cell growth when subjected to low concentration of copper and no-growth stimulation with low or moderate concentrations of cadmium. his finding resolves some of the confusion in the literature relative to the effect of low doses of cadmium.