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NEAR-BOTTOM PELAGIC BACTERIA AT A DEEP-WATER SEWAGE SLUDGE DISPOSAL SITE
Citation:
Takizawa, M., W. Straube, R. Hill, AND R. Colwell. NEAR-BOTTOM PELAGIC BACTERIA AT A DEEP-WATER SEWAGE SLUDGE DISPOSAL SITE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/005.
Description:
The epibenthic bacterial community at deep-ocean sewage sludge disposal site DWD-106, located approximately 106 miles (ca. 196 km) off the coast of New Jersey, was assessed for changes associated with the introduction of large amounts of sewage sludge. ixed cultures and bacterial isolates obtained from water overlying sediment core samples collected at the deep-water (2,500 m) municipal sewage disposal site were tested for the ability to grow under in situ conditions of temperature and pressure. he responses of cultures collected at a DWD-106 station heavily impacted by sewage sludge were compared with those of samples collected from a station at the same depth which was not contaminated by sewage sludge. ignificant differences were observed in the ability of mixed bacterial cultures and isolates from the two sites to grow under deep-sea pressure and temperature conditions. he levels of sludge contamination were established by enumerating Clostridium perfringens, a sewage indicator bacterium, n sediment samples from the two sites. he results of hybridization experiments in which DNAs extracted directly from the water overlying sediment core samples were used indicate that the reference site epibenthic community, the disposal site epibenthic community, and the community in a surface sludge plume share many members. ecreased culturability of reference site mixed cultures in the presence of sewage sludge was observed. hus, the culturable portions of both the autochthonous and allochthonous bacterial communities at the disposal site may be inhibited in situ, the former by sewage sludge and the latter by high pressure and low temperature.