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ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCHAROMYCES CEREVISIAE
Citation:
Chen, C., T. Turi, D. Sanglard, AND J. Loper. ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCHAROMYCES CEREVISIAE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/303.
Description:
We have isolated the gene for cytochrome P450 lanosterol 14a-demethylase (14DM) from the yeast Candida tropicalis. his was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. dentity of this gene was confirmed by a) observing a heme binding region common to all P450s after sequencing the 31 portion of the gene, and b) based upon tests of its expression in strains of Saccharomyces cerevisiae.
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ISOLATION OF THE CANDIDA TROPICALIS GENE FOR P450 LANOSTEROL DEMETHYLASE AND ITS EXPRESSION IN SACCHAROMYCES CEREVISIAERecord Details:
Record Type:DOCUMENT( REPORT )
Product Published Date:05/24/2002
Record Last Revised:04/16/2004
Record ID:
50731