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FUNCTIONAL TERATOGENS OF THE RAT KIDNEY II. NITROFEN AND ETHYLENETHIOUREA
Citation:
Daston, G., B. Rehnberg, B. Carver, AND R. Kavlock. FUNCTIONAL TERATOGENS OF THE RAT KIDNEY II. NITROFEN AND ETHYLENETHIOUREA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/538 (NTIS PB91109199).
Description:
Nitrofen and ethylenethiourea (ETU), agents known to prenatally induce hydronephrosis in rats, were assessed for their effects on postnatal renal functional maturation. oth were given by gavage to pregnant Sprague-Dawley rats on Gestation Day 11. itrofen was given at concentrations of 50 or 100 mg/kg and ETU at 0, 4O,or 60 mg/kg. enal function was examined in the offspring from birth until alter weaning, the period of renal functional maturation in the rat. aximal urine concentrating ability was measured alter DDAVP (desmopressin acetate, a vasopressin analog) challenge water deprivation. roximal tubule transport was measured in renal cortical slices. arious urinary parameters were measured. Both prenatal nitrofen and ETU exposure caused a large number of neonatal deaths at the high dose, and hydronephrotic was observed. he severity of the lesion increased with age. ydronephrotic animals were deficient in urine concentrating ability, which became more pronounced after weaning. A few other urinary parameters were altered, but cortical function appeared to he unaffected. Rats prenatally exposed to nitrofen, but with apparently normal kidneys, were significantly compromised in their ability to produce a concentrated urine in response to DDAVP challenge, on Postnatal 13 days (PDs) 6 and 14. y they were not different from controls in urine concentrating response. Rats prenatally exposed to the higher doses of ETU with grossly normal kidneys, had significantly plasma clearances of certain electrolytes in life, but by 27, they were not different from controls. roximal tubule transport of PAH was increased on PD 7 in ETU-exposed pups, but this effect did not persist.