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POTENTIAL FOR TRANSDUCTION OF PLASMIDS IN A NATURAL FRESHWATER ENVIRONMENT: EFFECT OF PLASMID DONOR CONCENTRATION AND A NATURAL MICROBIAL COMMUNITY ON TRANSDUCTION IN 'PSEUDOMONAS AERUGINOSA'
Citation:
Saye, D., O. Ogunseitan, G. Sayler, AND R. Miller. POTENTIAL FOR TRANSDUCTION OF PLASMIDS IN A NATURAL FRESHWATER ENVIRONMENT: EFFECT OF PLASMID DONOR CONCENTRATION AND A NATURAL MICROBIAL COMMUNITY ON TRANSDUCTION IN 'PSEUDOMONAS AERUGINOSA'. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-87/145.
Description:
The transduction of Pseudomonas aeruginosa plasmid Rms149 by the generalized transducing phage DS1 was shown to occur during a nine day incubation of environmental test chambers in a freshwater reservoir. Plasmid DNA was transferred from a nonlysogenic plasmid donor to a DS1 lysogen of Pseudomonas aeruginosa that served both as the source of the transducing phage and as the recipient of the plasmid DNA. Transduction of the plasmid in the presence of the natural microbial community of the reservoir was below the limits of detection employed. The results demonstrate that a potential exists for the transduction of plasmid DNA in aquatic habitats. (Copyright (c) 1987, American Society for Microbiology.)