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RECRUITMENT OF A CHROMOSOMALLY-ENCODED MALEYLACETATE REDYCTASE FOR THE DEGRADATION OF 2,4-DICHLOROPHENOXY-ACETIC ACID (TFD) BY PLASMID PJP4
Citation:
Kukor, J., R. Olsen, AND J. Siak. RECRUITMENT OF A CHROMOSOMALLY-ENCODED MALEYLACETATE REDYCTASE FOR THE DEGRADATION OF 2,4-DICHLOROPHENOXY-ACETIC ACID (TFD) BY PLASMID PJP4. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/436.
Description:
When Pseudomonas aeruginosa PAO1c or P. putida PP0220 or PP0300 carry plasmid pJP4, which encodes enzymes for the degradation of 2,4-dichlorophenoxyacetic acid (TFD) or 2-chloromaleylacetate, cells do not grow on TFD and UV-absorbing material with spectral characteristics of chloromaleylacetate accumulates in the culture medium. sing plasmid pR0l727, we cloned from the chromosome of a nonfluorescent pseudomonad, Pseudomonas sp. strain PK0l, 6- and 0.5-kilobase BamHI DNA fragments which contain the gene for maleylacetate reductase. ovel polypeptide with an estimated molecular weight of 18,000 was detected in cell extracts of P. aeruginosa carrying either plasmid pR0l944 or plasmid pR0945. Maleylacetate reductase activity was induced in cells of P putida carrying plasmid pR01945, as well as in cells in Pseudomonas strain PKOl, when grown on L-tyrosine, suggesting that the tyrosine catabolic pathway might be the source from which maleylacetate reductase is recruited for the degradation of TFD in pJP4-bearing cells of Pseudomonas sp. strain PK0l.