You are here:
GENETIC ORGANIZATION AND SEQUENCE OF THE PSEUDOMONAS CEPACIA GENES FOR THE ALPHA AND BETA SUBUNITS OF PROTOCATECHUATE 3,4-DIOXYGENASE
Citation:
Zylstra, G., R. Olsen, AND D. Ballou. GENETIC ORGANIZATION AND SEQUENCE OF THE PSEUDOMONAS CEPACIA GENES FOR THE ALPHA AND BETA SUBUNITS OF PROTOCATECHUATE 3,4-DIOXYGENASE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/426.
Description:
Locations of genes for a and B ubunits of protocatechuate 3,4-dioxygenase on a 9.5 kilobase pair fragment cloned from the Pseudomonas cepacia DBOl chromosome were determined after construction of several subclones into the broad-host-range cloning vectors pR023l7, pR02320, and pR02321. he ability of each subclone to complement mutations in protocatechuate 3,4-dioxygenase (pcaA) was tested in mutant strains derived from P. cepacia, P. aeruginosa, and P. putida. hese complementation studies also showed that the two subunits were expressed from the same promoter. he nucleotide sequence of the region encoding for protocatechuate 3,4-dioxygenase was determined. he deduced amino acid sequence matched that determined by N-terminal analysis of regions of the isolated enzyme. he deduced amino acid sequence shows extensive homology with the previously determined amino acid sequence of protocatechuate 3,4-dioxygenase from another Pseudomonas species.