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PHENOXYACETIC ACID DEGRADATION BY THE 2,4-DICHLOROPHENOXYACETIC ACID (TFD) PATHWAY OF PLASMID PJP4: MAPPING AND CHARACTERIZATION OF THE TFD REGULATORY GENE
Citation:
Harker, A., R. Olsen, AND R. Seidler. PHENOXYACETIC ACID DEGRADATION BY THE 2,4-DICHLOROPHENOXYACETIC ACID (TFD) PATHWAY OF PLASMID PJP4: MAPPING AND CHARACTERIZATION OF THE TFD REGULATORY GENE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/422.
Description:
Plasmid pJP4 enables Alcaligenes eutrophus JMP134 to degrade 3-chlorobenzoate and 2,4-dichlorophenoxyacetic acid (TFD), Plasmid pROl0 is a derivative of pJP4 obtained by insertion of TN1721 into a nonessential region of pJP4. lasmid pROl0l was transferred by conjugation to several Pseudomonas strains and to A. eutrophus AEO106, a cured isolate of JMP134. EOl06 (pROl0l) and some transconjugants grev on TFD. ransconjugants with a chromosomally encoded phenol hydroxylase also degraded phenoxyacetic acid (PAA) in the presence of an inducer of the TFD pathway, namely, TFD or 3-chlorobenzoate. utant of one such phenol-degrading strain, Pseudomonas putida PPO300 (pR) 101), grew on PAA as the sole carbon source in the absence of inducer. his isolate carried a mutant plasmid, designated pROl03, derived from pROl0l through the deletion of a 3.9-kilobase DNA fragment. lasmid pRO103 constitutively expressed the TFD pathway, and this allowed the metabolism of PAA in the absence or the inducer, TFD.