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COMPARISON OF BIOASSAY AND ENZYME-LINKED IMMUNOSORBENT ASSAY FOR QUANTIFICATION OF 'SPODOPTERA FRUGIPERDA' NUCLEAR POLYHEDROSIS VIRUS IN SOIL
Citation:
Kawanishi, C., J. Fuxa, AND G. Warren. COMPARISON OF BIOASSAY AND ENZYME-LINKED IMMUNOSORBENT ASSAY FOR QUANTIFICATION OF 'SPODOPTERA FRUGIPERDA' NUCLEAR POLYHEDROSIS VIRUS IN SOIL. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-85/396 (NTIS PB86202967).
Description:
Standard curves with known amounts of Spodoptera frugiperda nuclear polyhedrosis virus (NPV) in soil were established with a bioassay and with an enzyme-linked immunosorbent assay (ELISA). The bioassay detected as few as 4 x 10 to the 4th power polyhedral inclusion bodies (PIB)/g of sandy soil and < 10 PIB/g of soils with large amounts of silt or clay. The ELISA detected as few as 360 PIB/g in all three soil types, and absorbance values were inversely related to the amount of clay. Results of the bioassay and ELISA were significantly (P < 0.01) correlated for natural NPV from field samples of silt (R equal 0.961) and sandy soil (R=0.723). Soil samples from Louisiana pastures and corn fields contain up to 7.6 times 10 to the 4th power PIB/g, and 2 times 10 to the 4th power PIB/g are commonly present.