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SUMMARY REPORT: EVALUATION OF A NEW FLUOROMETRIC TECHNIQUE THAT USES HIGHLY SELECTIVE INTERFERENCE FILTERS FOR MEASURING CHLOROPHYLL IN THE PRESENCE OF CHLOROPHYLL B AND PHEOPIGMENTS
Citation:
Arar, E. SUMMARY REPORT: EVALUATION OF A NEW FLUOROMETRIC TECHNIQUE THAT USES HIGHLY SELECTIVE INTERFERENCE FILTERS FOR MEASURING CHLOROPHYLL IN THE PRESENCE OF CHLOROPHYLL B AND PHEOPIGMENTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/R-94/150 (NTIS PB94210622).
Description:
A new fluorometric technique was compared to conventional fluorometry with and without pheophytin a (pheo a) correction and to spectrophotometry using Lorenzen's modified monochromatic equations and Jeffrey and Humphrey's trichromatic equation to calculate chlorophyll a (chl a). he new technique uses excitation and emission filters that are highly selective for chl a, eliminating the interference caused by accessory pigments and pheopigment degradation products. our method parameters were evaluated using the new technique, conventional fluorometry and spectrophotometry. hey were (1) sensitivity, (2) linear dynamic range, (3) precision, and (4) accuracy. ontrolled studies of the interference caused by chl b were conducted, and real world samples of varying taxonomic composition were analyzed. or laboratory solutions, the new technique was comparable to conventional fluorometry-with respect to sensitivity and accuracy. he linear dynamic range for the new technique exceeded that of conventional fluorometry by a factor of three. nterference caused by chl b was +6% at the highest chl a:chl b likely to occur in nature. hl a values obtained using the new technique compared well with conventional fluorometry when pheo a was the only interfering pigment present in the sample.