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AMMONIA REMOVAL FROM MAMMALIAN CELL CULTURE MEDIUM
Citation:
Sikdar, S. AND S. Sawant. AMMONIA REMOVAL FROM MAMMALIAN CELL CULTURE MEDIUM. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/371 (NTIS PB95124863).
Description:
Metabolites such as ammonia and lactic formed during mammalian cell culture can frequently be toxic to the cells themselves beyond a threshold concentration of the metabolites. ell culture conducted in the presence of such accumulated metabolites is therefore limited in productivity. his work demonstrates with laboratory data that a non-porous ion exchange membrane of the perfluorinated sulfonic acid type can be used to contact the culture medium, and ammonia removed selectivity from the medium without disturbing the process. he technique of pervaporation showed particular promise in this regard. he pervaporation used with inert gas sweep on the permeate side was found superior to that used with vacuum application.