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DIFFERENCES IN ARACHIDONIC ACID METABOLISM BY HUMAN MYELOMONCYTIC CELL LINES
Citation:
Madden, M., S. Becker, H. Koren, AND M. Friedman. DIFFERENCES IN ARACHIDONIC ACID METABOLISM BY HUMAN MYELOMONCYTIC CELL LINES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/280 (NTIS PB92217215).
Description:
The production of arachidonic acid metabolites by the HL60, ML3, and U937 human phagocyte cell lines were determined after incubation with interferongamma (IFNg; 500 U/ml) or vehicle for 4 days. ells were prelabeled with tritiated arachidonic acid for 4 hours, and media supernatants analyzed by high performance liquid chromatography. one of the cell lines produced H-AA metabolites in large amounts during an unstimulated, basal release period (30 or 60 min). n response to 10 uM calcium ionophore A23187 incubation (30 min), undifferentiated and IFNg-differentiated HL60 cells formed both cyclooxygenase products thromboxane and prostaglandins) and lipoxygenase products (leukotrienes and hydroxyeicosatetraenoic acids). In contrast to the HL60 cells, IFNg-differentiated U937 cells formed primarily cyclooxygenase products, while undifferentiated and IFNg-differentiated ML3 cells did not form any 3H-AA metabolites in response to A23187. hese data point out the need to carefully select a cell line for use in a phagocyte assay system when cyclooxygenase and/or lipoxygenase products could influence the assay results.
