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AGE-RELATED CHANGES IN RECEPTOR-MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN VARIOUS REGIONS OF RAT BRAIN
Citation:
Mundy, W., P. Tandon, S. Ali, AND H. Tilson. AGE-RELATED CHANGES IN RECEPTOR-MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN VARIOUS REGIONS OF RAT BRAIN. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/210 (NTIS PB91243014).
Description:
The effects of age on cholinergic markers and receptor-stimulated phosphoinositide hydrolysis was dined in the frontal cortex and striatum of male Fischer-344 rats. holine acetyltransferase activity was decreased 27% in the striatum of aged (24 month) rats cared to young (3 month) controls. uscarinic receptor density as measured by [3H]-quinuclidinyl benzilate binding showed a solar 26% decrease in the striatum of aged rats. hosphoinositide hydrolysis was measured by the release of inositol phosphate (IP) from tissue slices prelabeled with [3H]myoinositol in response to carbachol, norepinephrine, and quisqualate. n the cortex, stimulated IP release was significantly greater in slices from aged rats compared to young rats for all three agonists. n contrast, stimulated IP release was significantly decreased in striatal slices from aged rats compared to young for all three agonists. hese data indicate a differential effect of age on agonist-stimulated phosphoinositide hydrolysis in the cortex and striatum. The ecreased responsiveness in the latter area may result from the age-related loss of postsynaptic receptors.