You are here:
IN VIVO AND IN VITRO CHARACTERISTICS OF EARLY CARCINOGEN-INDUCED PREMALIGNANT PHENOTYPES IN CULTURED RAT TRACHEAL EPITHELIAL CELLS (JOURNAL VERSION)
Citation:
Steele, V., J. Arnold, AND M. Mass. IN VIVO AND IN VITRO CHARACTERISTICS OF EARLY CARCINOGEN-INDUCED PREMALIGNANT PHENOTYPES IN CULTURED RAT TRACHEAL EPITHELIAL CELLS (JOURNAL VERSION). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/135.
Description:
The initial stages of neoplastic transformation in respiratory tract epithelial cells were defined and studied by characterizing a series of morphologically transformed cell colonies from carcinogen-exposed rat tracheal epithelial (TRE) cell cultures both in vivo and in vitro. RTE cells were isolated and exposed to 7,12-dimethylbenz(a)anthracene on Day 1 for 24 h. Between Days 26 and 30, single colonies of morphologically altered cells were isolated and classified into three major groups based on cell density. Class II and III cells exhibited a higher colony forming efficiency when replated on various substrates. Class III cells appeared to grow better than Class I or II cells under complete media conditions, while Class I cells grew better in media without 3T3 conditioning factors. At early passage levels, the population doubling times were longer for Class I cells, shorter for Class II cells, and even shorter for Class III cells. Inoculation of the three classes of cells into nude mice providing confirming evidence of the benign nature of Class I cell lines, and the malignant nature of some Class II cell lines and all of the Class III cell lines. (Copyright (c) IRL Press, Limited.)