You are here:
DNA ADDUCTS AND INDUCTION OF SISTER CHROMATID EXCHANGES IN THE RAT FOLLOWING BENZO[B]FLUORANTHENE ADMINISTRATION
Citation:
Ross, J., G. Nelson, K. Holden, G. Erexson, K. Earley, A. Beach, A. Kligerman, R. Gupta, AND S. Nesnow. DNA ADDUCTS AND INDUCTION OF SISTER CHROMATID EXCHANGES IN THE RAT FOLLOWING BENZO[B]FLUORANTHENE ADMINISTRATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/393.
Description:
Male Sprague Dawley rats were injected with single i.p. doses of benzo[a]pyrene (B[a]P), and peripheral blood lymphocytes (PBLs), livers, and lungs were removed at various times after administration. NA adducts, analyzed by 32P-postlabeling with nuclease P1 enhancement, were observed in all three tissues from 1 to 56 days after injection. aximal adduct levels occurred at about 4 days, followed by a gradual decrease. he DNA adducts induced by benzo[b]fluoranthene (B[b]F) were studied by a similar protocol with lungs, livers, and PBLs harvested from 1 to 56 days after i.p. injection of 100 mg/kg. everal DNA adducts were observed in each tissue with maximal levels occurring at about 7 days after treatment Lung DNA exhibited consistently higher adduct levels than DNA from liver or PBLS. ased upon our data, it appears that at least two differences in DNA adduction patterns may contribute to target tissue specificity for PAH carcinogenicity. n the case of B[a]P, qualitative differences between the adducts produced in different tissues are striking; while for B[b]F, quantitative differences in total levels and persistence of adducts may be more important.