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FRESHWATER ASSAY USING SOIL ELUATES AS SAMPLE MATERIAL (SINGLE LABORATORY EVALUATION)
Citation:
Lower, W., M. Thomas, B. Judy, AND W. Sutton. FRESHWATER ASSAY USING SOIL ELUATES AS SAMPLE MATERIAL (SINGLE LABORATORY EVALUATION). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/3-90/038 (NTIS PB90203456).
Description:
The Chlorophyta assay, which uses soil as sample material, has been a useful bioassessment technique for screening hazardous waste site problems. n eluate is prepared from a 125-gram soil sample and then diluted into three separate concentrations prior to being tested using Selenastrum capricornutum. he work reported here has attempted to determine the procedure's capability for data quality, to provide a basis for deciding whether the assay merits collaborative testing, and to define more clearly the method's potential for inclusion as part of an operational monitoring network. he soil used for most of this evaluation was a homogenized clay loam. oil containing either sodium fluoride or 2,4-dichlorophenol was tested using the algal assay, and several tests were conducted to confirm the dose/response curve using the positive control compound, zinc chloride. nown test response also was established using a white silica sand spiked with sodium fluoride. he known test response provides a value with a fairly high standard error, which, in some ways, probably detracts from the future application of this technique when accuracy and/or systematic error estimates are needed. esults revealed an apparently poor capability for sensitivity and a somewhat limited range of reliable measurement. he procedure, however, will definitely detect the presence of certain chemicals and, thus, can be used effectively as a screening technique.