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GENETIC VARIATION IN CLONAL VERTEBRATES DETECTED BY SIMPLE SEQUENCE FINGERPRINTING
Citation:
Turner, B., J. Elder, T. Laughlin, AND W. Davis. GENETIC VARIATION IN CLONAL VERTEBRATES DETECTED BY SIMPLE SEQUENCE FINGERPRINTING. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/384 (NTIS PB91163972).
Description:
Measurement of clonal heterogeneity is central to understanding evolutionary and population genetics of roughly 50 species of vertebrates lack effective genetic recombination. imple-sequence DNA fingerprinting with oligonucleotide probes (CAG)5 and (GACA)4 was used to detect heterogeneity in natural populations of two clonal fishes, Poecilia formosa, an apomictic unisexual, and Rivulus marmoratus, a selfing hermaphrodite. The technique clearly differentiates allozymically identical laboratory lines of marmoratus that were previously distinguishable only by histocompatibility analysis. he technique also revealed the first documented cases of apparent clonal turnover in a natural population of each species.
