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PRIMARY STRUCTURE OF THE P450 LANOSTEROL DEMETHYLASE GENE FROM SACCHAROMYCES CEREVISIAE
Citation:
Kalb, V., D. Woods, T. Turi, C. Dey, T. Sutter, AND J. Loper. PRIMARY STRUCTURE OF THE P450 LANOSTEROL DEMETHYLASE GENE FROM SACCHAROMYCES CEREVISIAE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/302 (NTIS PB93229243), 1987.
Description:
We have sequenced the structural gene and flanking regions for lanosterol 14oc-demethylase (14DM) from Saccharomyces cerevisiae. n open reading fram of 530 codons encodes a 60.7-kDa protein. hen this gene is disrupted by integrative transformation, the resulting strain requires ergosterol and, as expected, grows only in the absence of oxygen. he deduced amino acid sequence of 14DM includes a hydrophobic segment near the amino terminus which may be a transmembrane domain. he deduced sequence has been compared with those of eight other eukaryotic P450s, each from a different family within the P450 superfamily. hese comparisons indicate that this yeast P450 and several mammalian P450s have equivalent alignment scores when each is compared with the bovine P450scc. atrix comparisons of the amino acid sequence of this P450 with those of mammalian P450s reveal three conserved regions. he DNA region 51 to the structural 14DM gene includes poly(dA:dT) sequences and a repeating hexamer sequence.
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PRIMARY STRUCTURE OF THE P450 LANOSTEROL DEMETHYLASE GENE FROM SACCHAROMYCES CEREVISIAE