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BIOASSAY-DIRECTED FRACTIONATION OF THE ORGANIC EXTRACT OF SRM 1649 URBAN AIR PARTICULATE MATTER
Citation:
Lewtas, J., J. Chuang, M. Nishioka, AND B. Petersen. BIOASSAY-DIRECTED FRACTIONATION OF THE ORGANIC EXTRACT OF SRM 1649 URBAN AIR PARTICULATE MATTER. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/541 (NTIS PB91242511), 1990.
Description:
Separation of 2 grams of the methylene chloride extract of NIST SRM 1649 (Washington, D.C. urban air particulate matter) into six compound class fractions by acid-base partitioning and silica gel column chromatography is demonstrated here. ecoveries of organic mass and Salmonella typhimurium TA98 mutagenic activity were greater than 80 percent. he fractionation method showed reproducible distribution of both mass and mutagenic activity; the compound class mass distribution results were similar to those obtained for more typical analytical fractionation of a milligram quantity of the extract mass. he most polar compound class fractions contained the greatest proportions of mutagenic activity.