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COMPARISON OF EFFLUENT TOXICITY RESULTS USING CERIODAPHNIA DUBIA CULTURED ON SEVERAL DIETS
Citation:
Norberg-King, T. AND S. Schmidt. COMPARISON OF EFFLUENT TOXICITY RESULTS USING CERIODAPHNIA DUBIA CULTURED ON SEVERAL DIETS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/185 (NTIS PB94163649), 1993.
Description:
Several diets have been proposed for Ceriodaphnia dubia, but no single diet has been universally accepted as optimal for toxicity testing. lthough several diets for Ceriodaphnia dubia culturing and testing are commonly used, little or no data exist on whether toxicity varies with the diet. his study evaluated several combinations of yeast-Cerophyl-trout chow (YCT), Selenastrum capricornutum, and Selenastrum capricornutum-Cerophyl foods for routine culture performance and the sensitivity of the offspring in subsequent acute toxicity tests with effluents. ariations in the diets included use of a vitamin-fortified yeast added to the YCT, algae (Selenastrum capricornutum) grown in two different algal media, and different feeding rates of the algae-Cerophyl diets. leven diets were evaluated in a multi-generation feeding study, but only seven were used in subsequent toxicity tests. he young produced from each of the seven diets were tested in 48-h acute tests with three different effluents across the generations. oxicity tests with the effluents gave LC50s that were within a factor of two of one another, regardless of the food used for culturing. hese results indicate that several diets are satisfactory for culturing Ceriodaphnia dubia and that the results of the toxicity tests are comparable.