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STANDARD PRACTICE FOR CONDUCTING FUNGAL PATHOGENICITY TESTS ON THE LEPIDOPTERAN EGG PARASITE TRICHOGRAMMA PRETIOSUM
Citation:
Sewall, D. AND B. Lighthart. STANDARD PRACTICE FOR CONDUCTING FUNGAL PATHOGENICITY TESTS ON THE LEPIDOPTERAN EGG PARASITE TRICHOGRAMMA PRETIOSUM. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/3-90/069 (NTIS PB90263849), 1990.
Description:
As directed by Subdivision M of the Pesticide Assessment Guidelines, the entomogenous fungi being registered for use in the U.S. must be evaluated for nontarget effects on certain beneficial insects. ethods were developed and validated with the cosmopolitan entomogenous fungus Beauveria bassiana (ARSEF252) to evaluate the lethal effects of fungi on the beneficial wasp Trichogrmma pretiosum Riley via direct contact. . pretiosum challenged with B. bassiana (ARSEF252) produce dose responses with LC50's on day three of 10 5 to 10 6 and on day four of 10 4 to 10 5 conidia/ml. rocedures for conducting the bioassay, maintaining cultures of the insects used, and construction of special tools used in the bioassay are detailed.
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STANDARD PRACTICE FOR CONDUCTING FUNGAL PATHOGENICITY TESTS ON THE LEPIDOPTERAN EGG PARASITE TRICHOGRAMMA PRETIOSUM