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INTERACTIVE EFFECTS OF ALDRIN, CYCLOHEXYLAMINE, 2,4-DIAMINOTOLUENE AND TWO PHORBAL ESTERS ON METABOLIC COOPERATION BETWEEN V79 CELLS
Citation:
Mills, L.J., D. Robson, AND A. Malcolm. INTERACTIVE EFFECTS OF ALDRIN, CYCLOHEXYLAMINE, 2,4-DIAMINOTOLUENE AND TWO PHORBAL ESTERS ON METABOLIC COOPERATION BETWEEN V79 CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/233 (NTIS PB92108026), 1991.
Description:
The in vitro V79/metabolic cooperation assay measures the extent of gap-junctional transfer of metabolites from wild-type to mutant V79 cells. he assay is currently being explored as a short-term test to screen for tumor promoting chemicals, many of which inhibit metabolic cooperation. n this study, the assay was used to determine whether chemical interactions affect detection of tumor promoters in mixtures and to investigate types of interactions that may occur between chemicals. everal two-chemical mixtures were examined. he effects of phorbol-12-myristate-13-acetate (PMA) andphorbol-12,13-dibutyrate, two inhibitors of metabolic cooperation that operate through the same receptor-mediated pathway, were additive at concentrations below the maximally effective concentrations of either. ummation effect was observed in mixtures of two other inhibitors of metabolic cooperation, the pesticide aldrin and the principal metabolite of sodium cyclamate, cyclohexylamine.