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CYTOGENETIC STUDIES OF ETHYL ACRYLATE USING C57BL/6 MICE
Citation:
Kligerman, A., A. Atwater, M. Bryant, G. Erexson, P. Kwanyuen, AND K. Dearfield. CYTOGENETIC STUDIES OF ETHYL ACRYLATE USING C57BL/6 MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/090 (NTIS PB91200246), 1991.
Description:
The clastogenicity of ethyl acrylate (EA) was examined in vivo by injecting i.p. 5 male C57BL/6 mice per dose group with either 125, 250, 500, 1000 mg/kg EA dissolved in saline. wenty-four hours after injection, the animals were anesthetized, the spleens aseptically removed, and the splenic lymphocytes cultured for scoring chromosome aberrations (CAs) in first division cells and sister chromatid exchanges (SCEs) in second division cells. n the remaining cultures cytochalasin a was added to produce binucleated cells for scoring micronuclei (MN). here was no other significant increase in SCEs or CAs at any of the doses of EA examined. t the highest dose examined (1000 mg/kg), EA did cause a small but significant increase in binucleated cell MN. solated splenocytes were exposed to a wide range of concentrations of EA during stage of the cell cycle or 23 h after mitogen stimulation during the late G1 or early S-phrase of the cell cycle. lthough EA was toxic for both exposure regimes, significant increases in chromatid-type aberrations were found only when the target cells were treated 23 h after mitogenic stimulation. o statistically-significant increase in SCE frequency was found after either treatment regime. hese data suggest that EA is only clastogenic at near toxic concentrations during a specific stage of the cell cycle.