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SEED GERMINATION AND ROOT ELONGATION TOXICITY TESTS IN HAZARDOUS WASTE SITE EVALUATION: METHODS DEVELOPMENT AND APPLICATIONS
Citation:
Linder, G., J. Greene, H. Ratsch, J. Nwosu, S. Smith, AND D. Wilborn. SEED GERMINATION AND ROOT ELONGATION TOXICITY TESTS IN HAZARDOUS WASTE SITE EVALUATION: METHODS DEVELOPMENT AND APPLICATIONS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-89/109 (NTIS PB90113184), 1989.
Description:
Seed germination tests measure soil toxicity directly, while root elongation tests consider the indirect effects of water-soluble constituents which may be present in site-samples. n the seed germination toxicity test, site-soil is mixed with a reference soil to yield exposure concentrations into which test seeds are planted. ermination is evaluated after a five-day exposure, and effective concentrations associated with a 50% reduction in seed germination are calculated. oot elongation tests evaluate site-soil eluates. ere, seeds are placed onto moistened filter paper which lines Petri dish exposure chambers; exposure chambers are then covered and incubated in complete darkness for five days. nhibition of root elongation is calculated as an (exposure concentration which yields a 50% reduction in root length relative to controls) upon termination of the test.
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SEED GERMINATION AND ROOT ELONGATION TOXICITY TESTS IN HAZARDOUS WASTE SITE EVALUATION: METHODS DEVELOPMENT AND APPLICATIONS