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INDUCTION OF MICRONUCLEI BY X-RADIATION IN HUMAN, MOUSE, AND RAT PERIPHERAL BLOOD LYMPHOCYTES
Citation:
Erexson, G., A. Kligerman, M. Bryant, M. Sontag, AND E. Halperin. INDUCTION OF MICRONUCLEI BY X-RADIATION IN HUMAN, MOUSE, AND RAT PERIPHERAL BLOOD LYMPHOCYTES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/269 (NTIS PB92113315), 1991.
Description:
We compared the radiosensitivity of human, rat, and mouse peripheral blood lymphocytes (PBLs) by analyzing micronuclei (MN) in cytochalasin B-induced binucleated (BN) cells. or each species and dose, 4 ml aliquots of whole blood were X-irradiated to obtain doses of 38, 75, 150, or 300 cGy. ontrols were sham-irradiated. fter exposure to X-rays, mononuclear leukocytes were isolated using density gradients and cultured in RPMI 1640 medium containing phytohemagglutinin to stimulate mitogenesis. t 21 h cytochalasin 8 was added to produce BN PBLs, and all cultures were harvested at 52 h post initiation using a cytocentrifuge. ignificant dose-dependent increases in the percentage of micronucleated cells and the number of MN per BN cell were observed in all three species. he linear-quadratic regression curves for the total percentage of micronucleated cells for the three species were similar; however, the curve for the mouse PBLs had a larger quadratic component than either of the curves for the rat or PBLs. lthough the correlation between the percentage of cells with MN and those human the mouse and rat PBLs were over twice with chromosome aberrations was high as efficient as human PBLs in forming MN from presumed acentric fragments. hese data indicate that the induction of MN in BN cells following ionizing radiation is similar in human, rat, and mouse PBLs, but care must be taken in using the MN results to predict frequencies of cells with chromosomal aberrations.
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INDUCTION OF MICRONUCLEI BY X-RADIATION IN HUMAN, MOUSE, AND RAT PERIPHERAL BLOOD LYMPHOCYTES