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AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS EFFICIENTLY ENTERS BUT DOES NOT REPLICATE IN POIKILOTHERMIC VERTEBRATE CELLS
Citation:
Brusca, J., M. Summers, J. Couch, AND L. Courtney. AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS EFFICIENTLY ENTERS BUT DOES NOT REPLICATE IN POIKILOTHERMIC VERTEBRATE CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-86/428 (NTIS PB88149174), 1986.
Description:
The host range of the insect virus Autographa californica nuclear polyhedrosis virus (AcMNPV) was examined. AcMNPV could not initiate a productive infection in frog, turtle, trout, or moth cell lines. After exposure to AcMNPV, neither viral DNA nor RNA synthesis could be detected in these cell lines when assayed by dot-blot hybridizations. Entry of viral DNA to the nucleus, however, was as efficient in the nonpermissive cell lines as it was in a permissive insect cell line. Electron microscopy revealed numerous AcMNPV nucleocapsids in the cytoplasm of the nonpermissive cell lines is therefore at a stage subsequent to viral entry to the nucleus. (Copyright (c) 1986, Intervirology.)